5EO0

Crystal Structure of HLA-B0702-RFL9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.956
Spacegroup name	P 21 21 21
Unit cell lengths	50.837, 81.980, 110.768
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.370 - 1.700
R-factor	0.1682
Rwork	0.166
R-free	0.21210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.535
Data reduction software	iMOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.370	37.370	1.790
High resolution limit [Å]	1.700	5.380	1.700
Rmerge	0.647	0.086	7.345
Rmeas	0.699
Rpim	0.261	0.038	0.036
Total number of observations	362268	11906	52803
Number of reflections	51552
<I/σ(I)>	6.6	14.3	1.9
Completeness [%]	100.0	99.4	100
Redundancy	7	6.6	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	18% PEG 4000 and 0.1 M Ammonium acetate, 0.1 M Na-cacodylate