5ENW
Structure of HLA-A2:01 with peptide G9L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.474, 80.483, 57.075 |
| Unit cell angles | 90.00, 113.41, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.850 |
| R-factor | 0.21007 |
| Rwork | 0.208 |
| R-free | 0.24354 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mre |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.129 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.128 | 0.575 |
| Number of reflections | 35425 | |
| <I/σ(I)> | 21.5 | 3.1 |
| Completeness [%] | 93.2 | 96.1 |
| Redundancy | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 30% PEG 4000, 0.1M Tris-HCl pH 8.0, 0.2M lithium sulfate |
