5EN2
Molecular basis for antibody-mediated neutralization of New World hemorrhagic fever mammarenaviruses
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-26 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97890 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.110, 74.790, 177.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 68.930 - 1.821 |
R-factor | 0.1827 |
Rwork | 0.180 |
R-free | 0.22440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kas 3fmg |
RMSD bond length | 0.010 |
RMSD bond angle | 1.060 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER (2.5.5) |
Refinement software | BUSTER-TNT (BUSTER 2.10.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 68.930 | 68.930 | 1.920 |
High resolution limit [Å] | 1.821 | 5.760 | 1.820 |
Rmerge | 0.068 | 1.418 | |
Rmeas | 0.248 | ||
Rpim | 0.101 | 0.031 | 0.648 |
Total number of observations | 371895 | 12483 | 51454 |
Number of reflections | 63113 | ||
<I/σ(I)> | 6.7 | 16.4 | 1.5 |
Completeness [%] | 99.9 | 99.9 | 99.9 |
Redundancy | 5.9 | 5.6 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 298 | JUNV GP1 and GD01 Fab, when mixed in equimolar ratio crystallized in mother liquor containing 24% PEG 3350 (v/v), 10 mM Tris, pH 8.0, 150 mM NaCl. |