5EN1
Crystal structure of hnRNPA2B1 in complex with RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-26 |
| Detector | RIGAKU |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.623, 46.380, 37.516 |
| Unit cell angles | 90.00, 96.02, 90.00 |
Refinement procedure
| Resolution | 28.810 - 2.580 |
| R-factor | 0.19387 |
| Rwork | 0.192 |
| R-free | 0.23624 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u1q |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.615 |
| Data reduction software | DENZO |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.810 |
| High resolution limit [Å] | 2.580 |
| Number of reflections | 6187 |
| <I/σ(I)> | 7.9 |
| Completeness [%] | 91.9 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 25% PEG3350, 0.1M Tris pH8.5 |
