5EMX
Crystal structure of the S. cerevisiae Rtf1 histone modification domain mutant R124A R126A R128A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2011-07-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 94.271, 94.271, 76.842 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.626 - 1.399 |
| R-factor | 0.1436 |
| Rwork | 0.142 |
| R-free | 0.17800 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.004 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.340 |
| High resolution limit [Å] | 1.320 | 1.320 |
| Rmerge | 0.051 | 0.626 |
| Number of reflections | 30553 | |
| <I/σ(I)> | 77.79 | 1.3 |
| Completeness [%] | 98.3 | 78.5 |
| Redundancy | 13.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M phosphate-citrate (pH 4.2) and 40% PEG 300 |
