5EMW
Crystal structure of the palmitoylated human TEAD3 transcription factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.310, 123.530, 150.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 95.540 - 2.550 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5emv |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.674 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (0.5.9) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.540 | 2.660 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.118 | 0.691 |
| Number of reflections | 38149 | |
| <I/σ(I)> | 9.5 | 2.5 |
| Completeness [%] | 94.9 | 96.8 |
| Redundancy | 5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 292 | 100 mM Sodium Acetate, 100 mM Calcium acetate, 12% PEG4000 |
