5EMV

Crystal structure of the palmitoylated human TEAD2 transcription factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-06
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9787
Spacegroup name	I 1 2 1
Unit cell lengths	79.830, 61.250, 112.672
Unit cell angles	90.00, 101.89, 90.00

Refinement procedure

Resolution	53.543 - 2.000
R-factor	0.2034
Rwork	0.202
R-free	0.23130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3l15
RMSD bond length	0.003
RMSD bond angle	0.668
Data reduction software	MOSFLM
Data scaling software	SCALA (0.5.4)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	58.320	58.320	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.072	0.024	0.674
Rpim	0.043	0.016	0.405
Total number of observations	137128	1474	10185
Number of reflections	36163
<I/σ(I)>	9.9	26.2	2
Completeness [%]	100.0	98.7	100
Redundancy	3.8	3.4	3.8
CC(1/2)	0.998	0.996	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	292	1.7-2.1 M Sodium Formate