5EMP

Transcription factor GRDBD and mmGRE complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-10
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.100
Spacegroup name	P 21 21 21
Unit cell lengths	39.279, 100.492, 112.043
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.008 - 2.300
R-factor	0.195
Rwork	0.193
R-free	0.23800
RMSD bond length	0.008
RMSD bond angle	1.453
Data scaling software	XSCALE
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.584		2.500
High resolution limit [Å]	2.300	10.000	2.300
Rmerge	0.083	0.035	0.498
Rmeas	0.093	0.040	0.552
Total number of observations	104716
Number of reflections	20343	285	4435
<I/σ(I)>	11.77	26.51	3.17
Completeness [%]	99.4	96.3	100
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	50mM Tris, 200mM KCl, 50mM MgCl2, 10% PEG 4000