5EMO

Structure of the star domain of T-STAR in complex with AUUAAA RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97934
Spacegroup name	P 1 21 1
Unit cell lengths	51.601, 79.967, 53.831
Unit cell angles	90.00, 101.05, 90.00

Refinement procedure

Resolution	28.508 - 3.030
R-factor	0.2256
Rwork	0.221
R-free	0.26890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5el3
RMSD bond length	0.003
RMSD bond angle	0.669
Data reduction software	xia2
Data scaling software	Aimless (0.3.11)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.510	28.510	3.110
High resolution limit [Å]	3.030	13.540	3.030
Rmerge	0.114	0.028	0.482
Rpim	0.081	0.021	0.343
Total number of observations	20367	196	1395
Number of reflections	7908
<I/σ(I)>	8.1	33.6	1.9
Completeness [%]	94.8	78.5	90.3
Redundancy	2.6	2.8	2.6
CC(1/2)	0.988	0.997	0.804

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	NaCl, Na-HEPES, PEG 4000