5EMI
N-acetylmuramoyl-L-alanine amidase AmiC2 of Nostoc punctiforme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-08 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.030, 62.760, 65.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.246 - 1.120 |
R-factor | 0.1348 |
Rwork | 0.134 |
R-free | 0.14600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jwq |
RMSD bond length | 0.010 |
RMSD bond angle | 1.492 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.150 |
High resolution limit [Å] | 1.120 | 1.120 |
Number of reflections | 52924 | |
<I/σ(I)> | 31.54 | 3.65 |
Completeness [%] | 94.3 | 69.3 |
Redundancy | 11.8 | 6.58 |
CC(1/2) | 1.000 | 0.884 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 1 microL of protein (3.7 mg/ml) was mixed with 1 microL of well solution containing 120 mM alcohols (mixture of 1,6-hexanediol, 1-butanol, 1,2-propanediol, 2-propanol, 1,4-butanediol, and 1,3-propanediol), 100 mM MES/imidazole at pH 6.5, and 37.5 % PEG1000/PEG3350/MPD |