5EM3

Crystal structure of the human BRPF1 bromodomain in complex with SEED9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99987
Spacegroup name	P 32 2 1
Unit cell lengths	60.140, 60.140, 63.220
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.198 - 1.400
R-factor	0.1745
Rwork	0.174
R-free	0.18770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lc2
RMSD bond length	0.010
RMSD bond angle	0.780
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.200	1.480
High resolution limit [Å]	1.400	1.400
Rmerge	0.049	0.236
Number of reflections	26409
<I/σ(I)>	25.4	8.4
Completeness [%]	99.7
Redundancy	9.2	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Sodium-Acetate, pH5.5, 0.15 M Sodium Nitrate, 20% PEG3350