5ELK
Crystal structure of mouse Unkempt zinc fingers 4-6 (ZnF4-6), bound to RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-21 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.282800 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.192, 51.203, 37.460 |
| Unit cell angles | 90.00, 97.18, 90.00 |
Refinement procedure
| Resolution | 19.348 - 2.300 |
| R-factor | 0.1824 |
| Rwork | 0.180 |
| R-free | 0.22350 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.164 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 41.250 |
| High resolution limit [Å] | 2.290 |
| Number of reflections | 5787 |
| <I/σ(I)> | 16 |
| Completeness [%] | 96.7 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Hepes pH 7.0, 17 % PEG 3350 |
