5EL6

Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the first position and antibiotic paromomycin

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	208.400, 446.000, 617.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	151.238 - 3.100
R-factor	0.196
Rwork	0.196
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wq1
RMSD bond length	0.009
RMSD bond angle	1.619
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	200.000		3.200
High resolution limit [Å]	3.100	50.000	3.100
Rmerge	0.292	0.064	3.982
Rmeas	0.294	0.065	4.104
Total number of observations	55612977
Number of reflections	1031479	286	92684
<I/σ(I)>	12.42	87.04	0.9
Completeness [%]	99.9	91.1	99.5
Redundancy	53.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 20000, PEG 550mme, KSCN, Tris-OAc