5EKG

Crystallization and X-ray Diffraction Data Collection of Importin-alpha from Mus musculus Complexed with a XPG NLS Peptide, fragment 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.4
Spacegroup name	P 21 21 21
Unit cell lengths	78.492, 90.842, 100.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.933 - 2.800
R-factor	0.1896
Rwork	0.187
R-free	0.22810
RMSD bond length	0.010
RMSD bond angle	1.282
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.800
Number of reflections	17937
<I/σ(I)>	6.9
Completeness [%]	99.3
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.6-0.7 M sodium citrate, 10 mM DTT