5EJY
Structure of Dictyostelium Discoideum Myosin VII MyTH4-FERM MF1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97242 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.210, 90.540, 148.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.308 - 1.900 |
| R-factor | 0.1962 |
| Rwork | 0.194 |
| R-free | 0.22970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pvl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.966 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 69695 | |
| <I/σ(I)> | 18.36 | 2.27 |
| Completeness [%] | 99.3 | 97.1 |
| Redundancy | 5.9 | 5.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | 18% PEG 8000, 100 mM MES pH 6.0 |
