5EJS

Structure of Dictyostelium Discoideum Myosin VII MyTH4-FERM MF2 domain, mutant 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	40.170, 158.840, 194.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.910 - 2.700
R-factor	0.2069
Rwork	0.205
R-free	0.23540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ejr
RMSD bond length	0.010
RMSD bond angle	1.120
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (2.5.6)
Refinement software	BUSTER-TNT (BUSTER 2.10.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.510	2.870
High resolution limit [Å]	2.700	2.700
Number of reflections	35131
<I/σ(I)>	26.42	3.95
Completeness [%]	99.7	96.9
Redundancy	12.9	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	290	11.9% PEG 3350, 0.1M Bis-Tris Propane pH 7.5