5EJO

Crystal structure of the winged helix domain in Chromatin assembly factor 1 subunit p90

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 41 2 2
Unit cell lengths	59.655, 59.655, 97.408
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.832 - 2.750
R-factor	0.2004
Rwork	0.197
R-free	0.22990
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	2.258
Phasing software	SHELX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.750	2.750
Rmerge		0.657
Number of reflections	8556
<I/σ(I)>	67.7	8.37
Completeness [%]	98.0	96.8
Redundancy	19.4	20.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	0.1 M NaCl, 1.5 M (NH4)2SO4, 0.1 M BIS-Tris