5EJL

MrkH, A novel c-di-GMP dependence transcription regulatory factor.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 41 21 2
Unit cell lengths	68.556, 68.556, 179.457
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.540 - 2.300
R-factor	0.2185
Rwork	0.216
R-free	0.26130
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	0.975
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	19898
<I/σ(I)>	31.96
Completeness [%]	99.9
Redundancy	13.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	Ammonium sulfate, Sodium citrate tribasic dihydrate, Lithium sulfate monohydrate