5EJB
Crystal structure of prefusion Hendra virus F protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.99969 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 108.610, 163.500, 147.940 |
Unit cell angles | 90.00, 94.13, 90.00 |
Refinement procedure
Resolution | 49.620 - 3.200 |
R-factor | 0.2542 |
Rwork | 0.253 |
R-free | 0.28330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b9b |
RMSD bond length | 0.011 |
RMSD bond angle | 1.541 |
Data reduction software | XDS (January 10, 2014) |
Data scaling software | XDS (January 10, 2014) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.620 | 3.280 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.122 | 0.666 |
Number of reflections | 80411 | |
<I/σ(I)> | 8.37 | 1.9 |
Completeness [%] | 99.6 | 99.6 |
Redundancy | 3.99 | 4.07 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 100mM sodium acetate pH 5.0, 1.75M lithium sulfate, 100mM magnesium sulfate, 3.4 % isopropanol |