5EJ1
Pre-translocation state of bacterial cellulose synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-15 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.355, 218.230, 220.765 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.864 - 3.400 |
| R-factor | 0.2354 |
| Rwork | 0.233 |
| R-free | 0.27690 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.922 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.500 |
| High resolution limit [Å] | 3.400 |
| Number of reflections | 86075 |
| <I/σ(I)> | 9.6 |
| Completeness [%] | 99.8 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 300 | sodium acetate |
