5EIV
Crystal structure of complex of osteoclast-associated immunoglobulin-like receptor (OSCAR) and a synthetic collagen consensus peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.707, 136.585, 70.584 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 70.584 - 2.414 |
| R-factor | 0.1765 |
| Rwork | 0.174 |
| R-free | 0.22040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5eiq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.309 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 136.585 | 136.585 | 2.540 |
| High resolution limit [Å] | 2.414 | 7.630 | 2.410 |
| Rmerge | 0.048 | 0.680 | |
| Rmeas | 0.149 | ||
| Rpim | 0.057 | 0.022 | 0.321 |
| Total number of observations | 190535 | 6025 | 26139 |
| Number of reflections | 28164 | ||
| <I/σ(I)> | 11 | 28 | 2.3 |
| Completeness [%] | 99.9 | 99.8 | 100 |
| Redundancy | 6.8 | 6.5 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | Ammonium Sulphate, Sodium Citate, Glycerol, MPD |
