5EIU
Mini TRIM5 B-box 2 dimer C2 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-23 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.662, 41.488, 111.301 |
| Unit cell angles | 90.00, 99.06, 90.00 |
Refinement procedure
| Resolution | 32.475 - 1.908 |
| R-factor | 0.1846 |
| Rwork | 0.183 |
| R-free | 0.21710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.644 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.115 | |
| Number of reflections | 25826 | |
| <I/σ(I)> | 14 | 1.2 |
| Completeness [%] | 98.6 | 90.4 |
| Redundancy | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 0.1M Tris 7.0, 0.2M NaCl, 28% PEG3350, + (0.025% w/v Ala-ala, 0.025% w/v Ala-gly, 0.025% w/v Gly-gly-gly-gly, 0.025% w/v Leu-gly-gly from silver bullet) |
