5EIM

YTH domain-containing protein mmi1 and RNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-31
Detector	RIGAKU
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	53.105, 55.871, 104.541
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.540
R-factor	0.17447
Rwork	0.172
R-free	0.22461
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eip
RMSD bond length	0.019
RMSD bond angle	1.848
Data reduction software	Coot
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	52.270
High resolution limit [Å]	1.540
Number of reflections	43899
<I/σ(I)>	17.4
Completeness [%]	94.0
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	25% PEG3350, 8% Tacsimate