5EID
AKR2A ankyrin repeat domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.141, 39.193, 28.499 |
| Unit cell angles | 90.00, 96.29, 90.00 |
Refinement procedure
| Resolution | 37.354 - 2.000 |
| R-factor | 0.1895 |
| Rwork | 0.187 |
| R-free | 0.23490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hqd |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.063 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.110 | 0.514 |
| Number of reflections | 9423 | |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 97.0 | 97.6 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.06 M Ammonium sulfate, 0.05 M Sodium acetate trihydrate, 33% Polyethylene glycol 4000, 10% Glycerol |
