5EHM
Crystal structure of the Drosophila CG3822 KaiR1D ligand binding domain complex with NMDA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-23 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.000 |
Spacegroup name | P 31 |
Unit cell lengths | 56.891, 56.891, 143.584 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.810 - 1.281 |
R-factor | 0.1655 |
Rwork | 0.164 |
R-free | 0.19540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dtb |
RMSD bond length | 0.008 |
RMSD bond angle | 0.981 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1-2155_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.300 |
High resolution limit [Å] | 1.280 | 1.280 |
Rmerge | 0.035 | 0.370 |
Number of reflections | 134785 | |
<I/σ(I)> | 49.44 | 5.5 |
Completeness [%] | 96.2 | 70.1 |
Redundancy | 7.6 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | Buffer 150 NaCl 10 HEPES pH 7.5 20 mM NMDA 2 mM EDTA Reservoir 22% PEG 6K 0.1 M Na Acetate 0.1M Ammonium Sulfate |