5EHD

Crystal structure of human nucleophosmin-core in complex with cytochrome c

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-17
Detector	PSI PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	58.799, 179.720, 104.300
Unit cell angles	90.00, 94.32, 90.00

Refinement procedure

Resolution	45.526 - 2.550
R-factor	0.1668
Rwork	0.164
R-free	0.22910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2p1b
RMSD bond length	0.009
RMSD bond angle	1.265
Data reduction software	iMOSFLM
Data scaling software	Aimless
Refinement software	PHENIX ((1.10_2155: 000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.530	2.610
High resolution limit [Å]	2.550	2.550
Rmerge	0.126	0.048
Number of reflections	69833
<I/σ(I)>	9.6
Completeness [%]	99.6
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	25% PEG 400, 0.1 M MES, 0.1 M MgCl2