5EH8

human carbonic anhydrase II in complex with ligand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-09
Detector	ADSC QUANTUM 315
Wavelength(s)	0.99
Spacegroup name	P 1 21 1
Unit cell lengths	42.023, 41.424, 72.039
Unit cell angles	90.00, 104.21, 90.00

Refinement procedure

Resolution	40.737 - 1.381
R-factor	0.1512
Rwork	0.150
R-free	0.17330
RMSD bond length	0.010
RMSD bond angle	1.345
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	41.400
High resolution limit [Å]	1.380
Number of reflections	49161
<I/σ(I)>	12.2
Completeness [%]	99.3
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		280	ammonium sulfate, tris chloride