5EH6
Crystal Structure of the Glycophorin A Transmembrane Monomer in Lipidic Cubic Phase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 31.312, 31.312, 136.287 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.596 - 1.918 |
| R-factor | 0.2254 |
| Rwork | 0.224 |
| R-free | 0.23830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wol |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.890 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.600 | 1.986 |
| High resolution limit [Å] | 1.918 | 1.918 |
| Rmerge | 0.108 | 0.940 |
| Number of reflections | 2141 | |
| <I/σ(I)> | 9.91 | 2.37 |
| Completeness [%] | 97.6 | 93.61 |
| Redundancy | 9.6 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7.5 | 293 | 0.1 M HEPES, 20% PEG8000, 10 mM Tris, 40 mM NaCl |
