5EH5
human carbonic anhydrase II in complex with ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.010, 41.166, 71.816 |
| Unit cell angles | 90.00, 104.07, 90.00 |
Refinement procedure
| Resolution | 39.622 - 1.207 |
| R-factor | 0.1714 |
| Rwork | 0.171 |
| R-free | 0.18670 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.426 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.622 |
| High resolution limit [Å] | 1.207 |
| Number of reflections | 72887 |
| <I/σ(I)> | 23.2 |
| Completeness [%] | 99.4 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 280 | ammonium sulfate, tris-chloride |
