5EH4
Crystal Structure of the Glycophorin A Transmembrane Dimer in Lipidic Cubic Phase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 43.195, 43.195, 129.419 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.937 - 2.810 |
| R-factor | 0.2323 |
| Rwork | 0.228 |
| R-free | 0.25980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5eh6 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.638 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.940 | 2.910 |
| High resolution limit [Å] | 2.810 | 2.810 |
| Rmerge | 0.360 | 1.862 |
| Number of reflections | 3581 | |
| <I/σ(I)> | 14.6 | 3.1 |
| Completeness [%] | 99.5 | 97.4 |
| Redundancy | 12.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 20% (w/v)PEG 8000, 0.1 M sodium HEPES pH 7.5 10 mM TRIS-HCl pH 8, 40 mM NaCl |
