5EH1
Crystal structure of the extracellular part of receptor 2 of human interferon gamma
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-29 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.918409 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 58.102, 58.102, 377.266 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 62.880 - 1.800 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.525 |
| Data reduction software | XDS |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.880 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.799 |
| Number of reflections | 36722 | |
| <I/σ(I)> | 17.6 | 2.5 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 18.8 | 19.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1 M MES pH 5.0 + 10% PEG 6000, cryoprotected in 20% glycerol |
