5EGT
Crystal structure of Staphylococcal nuclease variant Delta+PHS V66E at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2015-10-07 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.178, 60.353, 38.425 |
| Unit cell angles | 90.00, 93.52, 90.00 |
Refinement procedure
| Resolution | 38.350 - 1.850 |
| R-factor | 0.1746 |
| Rwork | 0.172 |
| R-free | 0.21790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.815 |
| Data reduction software | CrysalisPro (1.171.36.32) |
| Data scaling software | CrysalisPro (1.171.36.32) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.022 | 0.118 |
| Number of reflections | 12215 | |
| <I/σ(I)> | 44.4 | 6.56 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 20% MPD, 25mM potassium phosphate, calcium chloride, pdTp |
