5EG7

The cap binding site of influenza virus protein PB2 as a drug target

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-26
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	45.262, 45.262, 156.451
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.356 - 1.400
R-factor	0.1627
Rwork	0.162
R-free	0.18340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eg8
RMSD bond length	0.025
RMSD bond angle	2.631
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	31.356
High resolution limit [Å]	1.400
Number of reflections	31468
<I/σ(I)>	2.3
Completeness [%]	99.8
Redundancy	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	10% PEG 3350