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5EG7

The cap binding site of influenza virus protein PB2 as a drug target

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 22-ID
Synchrotron siteAPS
Beamline22-ID
Temperature [K]100
Detector technologyCCD
Collection date2015-10-26
DetectorMARMOSAIC 300 mm CCD
Wavelength(s)1
Spacegroup nameP 43 21 2
Unit cell lengths45.262, 45.262, 156.451
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution31.356 - 1.400
R-factor0.1627
Rwork0.162
R-free0.18340
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)5eg8
RMSD bond length0.025
RMSD bond angle2.631
Data reduction softwareHKL-2000
Data scaling softwareSCALA
Phasing softwareMOLREP
Refinement softwareREFMAC (5.8.0049)
Data quality characteristics
 Overall
Low resolution limit [Å]31.356
High resolution limit [Å]1.400
Number of reflections31468
<I/σ(I)>2.3
Completeness [%]99.8
Redundancy10.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION6.529310% PEG 3350

229380

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