5EG7
The cap binding site of influenza virus protein PB2 as a drug target
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-26 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 45.262, 45.262, 156.451 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.356 - 1.400 |
R-factor | 0.1627 |
Rwork | 0.162 |
R-free | 0.18340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5eg8 |
RMSD bond length | 0.025 |
RMSD bond angle | 2.631 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 31.356 |
High resolution limit [Å] | 1.400 |
Number of reflections | 31468 |
<I/σ(I)> | 2.3 |
Completeness [%] | 99.8 |
Redundancy | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 10% PEG 3350 |