5EFC

Structure of Influenza B Lee PB2 cap-binding domain bound to GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-06-03
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.542
Spacegroup name	P 21 21 21
Unit cell lengths	40.515, 46.930, 87.952
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.798 - 1.900
R-factor	0.1851
Rwork	0.183
R-free	0.22660
RMSD bond length	0.007
RMSD bond angle	1.155
Data scaling software	Aimless (3.3.20)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.930	46.930	2.000
High resolution limit [Å]	1.900	6.010	1.900
Rmerge	0.120	0.039	0.714
Rpim	0.062	0.021	0.385
Total number of observations	63046	2058	8468
Number of reflections	13781
<I/σ(I)>	10.2	24.6	2.1
Completeness [%]	99.8	97.9	99.9
Redundancy	4.6	4.2	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	42% PEG400, 0.1 M Hepes pH 7.5