5EER

Crystal structure of DapB from Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-31
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	I 41 2 2
Unit cell lengths	107.386, 107.386, 175.669
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.2465
Rwork	0.244
R-free	0.29720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yl5
RMSD bond length	0.015
RMSD bond angle	1.888
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	14554
<I/σ(I)>	13.9
Completeness [%]	84.5	69.9
Redundancy	5.2	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Ammonium sulfate, 2-propanol