5EEA
Structure of HOXB13-DNA(CAA) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-06 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.972420 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 77.356, 57.918, 101.278 |
| Unit cell angles | 90.00, 101.57, 90.00 |
Refinement procedure
| Resolution | 45.945 - 2.195 |
| R-factor | 0.2521 |
| Rwork | 0.251 |
| R-free | 0.29650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5edn |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.264 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.020 | 2.310 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.143 | 1.135 |
| Number of reflections | 44173 | |
| <I/σ(I)> | 5.9 | 1.2 |
| Completeness [%] | 97.5 | 90.4 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG 5000, potassium chloride, magnesium chloride, PEG 400 |
