5EE0
Crystal structure of OsYchF1 at pH 6.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2013-05-11 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.618, 66.930, 66.945 |
| Unit cell angles | 90.00, 112.67, 90.00 |
Refinement procedure
| Resolution | 32.840 - 2.200 |
| R-factor | 0.236 |
| Rwork | 0.235 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ohf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.127 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.210 |
| Rmerge | 0.077 | 0.590 |
| Number of reflections | 23625 | |
| <I/σ(I)> | 19 | |
| Completeness [%] | 97.3 | 99.2 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG, 0.2M MGCL2, PH 6.5 |
