5EDJ
Crystal structure of the Neisseria meningitidis iron-regulated outer membrane lipoprotein FrpD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-23 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 64 |
| Unit cell lengths | 115.390, 115.390, 38.812 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.650 - 2.300 |
| R-factor | 0.18619 |
| Rwork | 0.183 |
| R-free | 0.24931 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5edf |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.267 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.071 | |
| Number of reflections | 13324 | |
| <I/σ(I)> | 22.9 | |
| Completeness [%] | 99.3 | 95.1 |
| Redundancy | 6.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris-HCl, 2 M ammonium sulfate |
