5ED9
Crystal structure of CC1 of mouse SUN2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.371, 34.743, 99.839 |
| Unit cell angles | 90.00, 101.49, 90.00 |
Refinement procedure
| Resolution | 36.295 - 2.009 |
| R-factor | 0.2134 |
| Rwork | 0.211 |
| R-free | 0.25690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | REFMAC ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.163 | 0.580 |
| Number of reflections | 19246 | |
| <I/σ(I)> | 9.1 | 3.4 |
| Completeness [%] | 98.6 | 97.9 |
| Redundancy | 5.4 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.1M Tris-HCl, 25%(w/v) PEG3350 |
