5ED7

Crystal Structure of HSV-1 UL21 C-terminal Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-15
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	54.273, 54.273, 180.757
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.980 - 2.720
R-factor	0.2184
Rwork	0.218
R-free	0.23430
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.408
Data reduction software	XDS
Data scaling software	SCALA (0.1.27)
Phasing software	SHELX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	54.270	54.270	2.850
High resolution limit [Å]	2.720	9.020	2.720
Rmerge	0.071	0.018	0.923
Rpim	0.029	0.008	0.364
Total number of observations	52609	1389	7173
Number of reflections	7854
<I/σ(I)>	20.6	66.5	2.4
Completeness [%]	99.7	99.6	99.7
Redundancy	6.7	5.2	7.2
CC(1/2)	0.999	1.000	0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 3350, sodium citrate, NDSB 256, sodium fluoride