5ECY
Structure of the Shigella flexneri VapC mutant D98N crystal form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9795 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 139.440, 139.440, 130.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.670 - 2.000 |
R-factor | 0.1805 |
Rwork | 0.180 |
R-free | 0.21380 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.072 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_1839) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.670 |
High resolution limit [Å] | 2.000 |
Number of reflections | 8429 |
<I/σ(I)> | 8.71 |
Completeness [%] | 100.0 |
Redundancy | 9.76 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292.15 | 2.8 M sodium acetate trihydrate |