5ECI
Crystal Structure of FIN219-FIP1 complex with JA, ATP and Mg
Experimental procedure
| Experimental method | LAUE |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-08 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97620-0.97622 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.889, 53.858, 195.396 |
| Unit cell angles | 92.52, 97.02, 113.58 |
Refinement procedure
| Resolution | 24.568 - 1.560 |
| R-factor | 0.2131 |
| Rwork | 0.212 |
| R-free | 0.22480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4epl |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.596 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.620 |
| High resolution limit [Å] | 1.560 | 3.360 | 1.560 |
| Rmerge | 0.088 | 0.054 | 0.726 |
| Total number of observations | 1043208 | ||
| Number of reflections | 275166 | ||
| <I/σ(I)> | 6.7 | ||
| Completeness [%] | 96.6 | 98.1 | 94.9 |
| Redundancy | 3.8 | 3.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 297 | 0.2 M sodium acetate trihydrate, 0.1 M Tris-HCl, 30%(w/v) PEG 3000 |
