5EC0

Crystal Structure of Actin-like protein Alp7A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.11587
Spacegroup name	P 21 21 21
Unit cell lengths	50.865, 76.153, 112.323
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.580 - 2.200
R-factor	0.1744
Rwork	0.169
R-free	0.22830
RMSD bond length	0.008
RMSD bond angle	1.148
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MR-Rosetta
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.580	2.278
High resolution limit [Å]	2.200	2.200
Number of reflections	22663
<I/σ(I)>	9.5	1.25
Completeness [%]	99.3	95.19
Redundancy	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298.15	0.16 M magnesium acetate, 0.08 M sodium cacodylate pH 6.5, 16% w/v PEG 8000, 20% v/v glycerol, Hampton Research Silver Bullets 22-HR2-996-22