5EBU
Aerococcus viridans L-lactate oxidase Y215F mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.346, 119.184, 119.560 |
| Unit cell angles | 90.00, 107.52, 90.00 |
Refinement procedure
| Resolution | 39.750 - 2.600 |
| R-factor | 0.18382 |
| Rwork | 0.182 |
| R-free | 0.24596 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.502 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.077 | 0.323 |
| Number of reflections | 87748 | |
| <I/σ(I)> | 14.7 | 4.8 |
| Completeness [%] | 99.3 | 96.8 |
| Redundancy | 3.75 | 3.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 30% PEG, 50 mM Tris-HCl, 50 mM pyruvate |
