5EBD
Crystal structure of EccB1 of Mycobacterium tuberculosis in spacegroup P21 (state IV)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-09-26 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 30.810, 111.160, 62.740 |
Unit cell angles | 90.00, 103.07, 90.00 |
Refinement procedure
Resolution | 41.119 - 2.600 |
R-factor | 0.2256 |
Rwork | 0.219 |
R-free | 0.28250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3x3m |
RMSD bond length | 0.004 |
RMSD bond angle | 0.933 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.580 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.061 | 0.451 |
Number of reflections | 9779 | |
<I/σ(I)> | 11.9 | 2.5 |
Completeness [%] | 77.8 | 73.2 |
Redundancy | 3.3 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 20mM Tris-Cl pH 7.5, 100mM magnesium formate, 15% (w/v) PEG 3350 |