5EBC
Crystal structure of EccB1 of Mycobacterium tuberculosis in spacegroup P21 (state III)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-08-26 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.610, 120.210, 61.260 |
| Unit cell angles | 90.00, 102.92, 90.00 |
Refinement procedure
| Resolution | 30.277 - 3.000 |
| R-factor | 0.237 |
| Rwork | 0.232 |
| R-free | 0.28630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3x3m |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.856 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.100 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.066 | 0.532 |
| Number of reflections | 8613 | |
| <I/σ(I)> | 10.3 | 2.5 |
| Completeness [%] | 96.1 | 88.9 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 20 mM Tris-Cl pH 7.5, 100 mM magnesium formate, 15% (w/v) PEG 3350 |
