5EB3

VB6-bound protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97861
Spacegroup name	P 43 21 2
Unit cell lengths	120.236, 120.236, 84.993
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.081 - 2.400
R-factor	0.1791
Rwork	0.178
R-free	0.19810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yn7
RMSD bond length	0.007
RMSD bond angle	1.021
Data reduction software	d*TREK
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.092	0.056	0.570
Total number of observations	173456
Number of reflections	24776
<I/σ(I)>	5.5
Completeness [%]	99.1	97.1	98.8
Redundancy	7	6.6	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1 M HEPES, 1.8 M ammonium sulfate