5EB2
Trp-bound YfiR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 120.875, 120.875, 88.457 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.535 - 2.709 |
| R-factor | 0.1965 |
| Rwork | 0.195 |
| R-free | 0.22990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yn7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.048 |
| Data reduction software | d*TREK |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 7.320 | 2.700 |
| Rmerge | 0.117 | 0.071 | 0.584 |
| Total number of observations | 527498 | ||
| Number of reflections | 18400 | ||
| <I/σ(I)> | 9.8 | ||
| Completeness [%] | 99.9 | 98.8 | 100 |
| Redundancy | 28.7 | 24.8 | 29.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.2 M NaCl, 0.1 M BIS-TRIS, 25%(w/v) polyethylene glycol 3350 |
