5EAY

Crystal structure of a Dna2 peptide in complex with Rpa 70N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	134.303, 50.882, 76.528
Unit cell angles	90.00, 103.94, 90.00

Refinement procedure

Resolution	30.000 - 1.550
R-factor	0.2327
Rwork	0.232
R-free	0.26670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b29
RMSD bond length	0.007
RMSD bond angle	1.329
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.000	60.000	1.550
High resolution limit [Å]	1.500	3.230	1.500
Rmerge	0.074	0.031	0.801
Rmeas	0.091	0.038	0.970
Rpim	0.051	0.022	0.541
Total number of observations	184727
Number of reflections	67556
<I/σ(I)>	16.7
Completeness [%]	84.3	93.8	79.7
Redundancy	2.7	2.8	2.7
CC(1/2)		0.997	0.525

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	50 mM Tris-HCl, 35 % PEG 1500, 2 mM TCEP, pH 8.0