5EAW

Crystal structure of Dna2 nuclease-helicase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	120.900, 148.600, 170.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.000
R-factor	0.2102
Rwork	0.209
R-free	0.24680
RMSD bond length	0.011
RMSD bond angle	1.700
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.110
High resolution limit [Å]	3.000	6.460	3.000
Rmerge	0.131	0.038	0.787
Rmeas	0.149	0.043	0.897
Rpim	0.068	0.020	0.419
Total number of observations	274504
Number of reflections	61754
<I/σ(I)>	4.1
Completeness [%]	98.3	96.7	98.9
Redundancy	4.4	4.3	4.5
CC(1/2)		0.999	0.708

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	80 mm MES pH 6.5, 250 mM Li2SO4, 8-12% PEG MME 5000, 2 mM MgCl2